Purification of phosphatidylglycerophosphate synthase from Chinese hamster ovary cells

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Purification of phosphatidylglycerophosphate synthase from Chinese hamster ovary cells.

Phosphatidylglycerophosphate (PGP) synthase catalyses the committed step in the biosynthesis of phosphatidylglycerol and cardiolipin in mammalian cells. Recently we isolated a Chinese hamster ovary (CHO) PGS1 cDNA encoding PGP synthase. In the present study we purified this PGP synthase to near-homogeneity from the mitochondrial fraction of CHO-K1 cells; the final enzyme preparation gave a sing...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2001

ISSN: 0264-6021

DOI: 10.1042/0264-6021:3540009